Open AccessGolgi/plastid‐type manganese superoxide dismutase involved in heat‐stress tolerance during grain filling of rice
Author(s) -
Shiraya Takeshi,
Mori Taiki,
Maruyama Tatsuya,
Sasaki Maiko,
Takamatsu Takeshi,
Oikawa Kazusato,
Itoh Kimiko,
Kaneko Kentaro,
Ichikawa Hiroaki,
Mitsui Toshiaki
Publication year - 2015
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12314
Subject(s) - biology , plastid , oryza sativa , golgi apparatus , superoxide dismutase , reactive oxygen species , genetically modified rice , heat stress , microbiology and biotechnology , transgene , biochemistry , botany , genetically modified crops , endoplasmic reticulum , enzyme , gene , chloroplast , zoology
Summary Superoxide dismutase ( SOD ) is widely assumed to play a role in the detoxification of reactive oxygen species caused by environmental stresses. We found a characteristic expression of manganese SOD 1 ( MSD 1 ) in a heat‐stress‐tolerant cultivar of rice ( Oryza sativa ). The deduced amino acid sequence contains a signal sequence and an N ‐glycosylation site. Confocal imaging analysis of rice and onion cells transiently expressing MSD 1‐ YFP showed MSD 1‐ YFP in the Golgi apparatus and plastids, indicating that MSD 1 is a unique Golgi/plastid‐type SOD . To evaluate the involvement of MSD 1 in heat‐stress tolerance, we generated transgenic rice plants with either constitutive high expression or suppression of MSD 1 . The grain quality of rice with constitutive high expression of MSD 1 grown at 33/28 °C, 12/12 h, was significantly better than that of the wild type. In contrast, MSD 1 ‐knock‐down rice was markedly susceptible to heat stress. Quantitative shotgun proteomic analysis indicated that the overexpression of MSD 1 up‐regulated reactive oxygen scavenging, chaperone and quality control systems in rice grains under heat stress. We propose that the Golgi/plastid MSD 1 plays an important role in adaptation to heat stress.