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PuHox52‐mediated hierarchical multilayered gene regulatory network promotes adventitious root formation in Populus ussuriensis
Author(s) -
Wei Ming,
Liu Quangang,
Wang Zhanchao,
Yang Jingli,
Li Wenlong,
Chen Yingxi,
Lu Han,
Nie Jinfu,
Liu Baoguang,
Lv Kaiwen,
Mao Xuliang,
Chen Su,
Sanders Jennifer,
Wei Hairong,
Li Chenghao
Publication year - 2020
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.16778
Subject(s) - auxin , cutting , biology , microbiology and biotechnology , homeobox , downregulation and upregulation , gene , gene regulatory network , regulation of gene expression , jasmonate , histone , gene expression , botany , genetics , arabidopsis , mutant
Summary Adventitious root (AR) formation is critically important in vegetative propagation through cuttings in some plants, especially woody species. However, the underlying molecular mechanisms remain elusive. Here, we report the identification of a poplar homeobox gene, PuHox52 , which was induced rapidly (within 15 min) at the basal ends of stems upon cutting and played a key regulatory role in adventitious rooting. We demonstrated that overexpression of PuHox52 significantly increased the number of ARs while suppression of PuHox52 had the opposite effect. A multilayered hierarchical gene regulatory network (ML‐hGRN) mediated by PuHox52 was reverse‐engineered and demonstrated to govern AR formation. PuHox52 regulated AR formation through upregulation of nine hub regulators, including a jasmonate signaling pathway gene, PuMYC2 , and an auxin signaling pathway gene, PuAGL12 . We also identified coherent type 4 feed‐forward loops within this ML‐hGRN; PuHox52 repressed PuHDA9 , which encodes a histone deacetylase, and led to an increase in acetylation and presumably expression of three hub regulators, PuWRKY51 , PuLBD21 and PuIAA7 . Our results indicate that the ML‐hGRN mediated by PuHox52 governs AR formation at the basal ends of stem cuttings from poplar trees.