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Several MYB transcription factors are known to play important roles in plant resistance to environmental stressors. However, the mechanism governing the involvement of MYBs in regulating tobacco mosaic virus (TMV) resistance in plants is still unclear. In this study, we found that not only is  Nicotiana benthamiana MYB4‐like  involved in defence against TMV, but also that the ethylene pathway participates in  MYB4L ‐mediated resistance. Transcription of  NbMYB4L  was up‐regulated in  N. benthamiana  infected with TMV. Silencing of  NbMYB4L  led to intensified TMV replication, whereas overexpression of  NbMYB4L  induced significant resistance to TMV. Transcription of  NbMYB4L  was greater in 1‐aminocyclopropanecarboxylic acid (ACC, ethylene precursor)‐pretreated plants but lower when the ethylene signalling pathway was blocked during TMV infection. Gene expression analysis showed that the transcription of  NbMYB4L  was largely suppressed in  ETHYLENE INSENSITIVE 3‐like 1(EIL1)‐ silenced plants. The results of electrophoretic mobility shift assay and chromatin immunoprecipitation‐quantitative PCR (ChIP‐qPCR) experiments indicated that NbEIL1 could directly bind to two specific regions of the  NbMYB4L  promoter. Furthermore, a luciferase assay revealed that NbEIL1 significantly induced the reporter activity of the  MYB4L  promoter in  N .  benthamiana . These results point to NbEIL1 functioning as a positive regulator of  NbMYB4L  transcription in  N. benthamiana  against TMV. Collectively, our work reveals that EIL1 and MYB4L constitute a coherent feed‐forward loop involved in the robust regulation of resistance to TMV in  N. benthamiana .

Ethylene‐induced NbMYB4L is involved in resistance against tobacco mosaic virus in Nicotiana benthamiana