Open AccessTwinfilin regulates actin assembly and Hexagonal peroxisome 1 (Hex1) localization in the pathogenesis of rice blast fungus Magnaporthe oryzae
Author(s) -
Xu Rui,
Li YuanBao,
Liu Chengyu,
Shen Ningning,
Zhang Qian,
Cao Tingyan,
Qin Minghui,
Han LiBo,
Tang Dingzhong
Publication year - 2021
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.13136
Subject(s) - biology , microbiology and biotechnology , hypha , actin , fungus , mutant , virulence , peroxisome , pathogenesis , botany , biochemistry , gene , immunology
Actin assembly at the hyphal tip is key for polar growth and pathogenesis of the rice blast fungus Magnaporthe oryzae . The mechanism of its precise assemblies and biological functions is not understood. Here, we characterized the role of M. oryzae Twinfilin (MoTwf) in M . oryzae infection through organizing the actin cables that connect to Spitzenkörper (Spk) at the hyphal tip. MoTwf could bind and bundle the actin filaments. It formed a complex with Myosin2 (MoMyo2) and the Woronin body protein Hexagonal peroxisome 1 (MoHex1). Enrichment of MoMyo2 and MoHex1 in the hyphal apical region was disrupted in a Δ Motwf loss‐of‐function mutant, which also showed a decrease in the number and width of actin cables. These findings indicate that MoTwf participates in the virulence of M. oryzae by organizing Spk‐connected actin filaments and regulating MoHex1 distribution at the hyphal tip.