Open AccessRedox responses of Arabidopsis thaliana to the green peach aphid, Myzus persicae
Author(s) -
Xu Junhuan,
Padilla Carmen S.,
Li Jiamei,
Wickramanayake Janithri,
Fischer Hillary D.,
Goggin Fiona L.
Publication year - 2021
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/mpp.13054
Subject(s) - myzus persicae , biology , aphid , arabidopsis thaliana , arabidopsis , reactive oxygen species , phloem , peroxisome , botany , apoplast , superoxide , lipaphis erysimi , biochemistry , mutant , gene , cell wall , enzyme
The green peach aphid ( Myzus persicae ) is a phloem‐feeding insect that causes economic damage on a wide array of crops. Using a luminol‐based assay, a superoxide‐responsive reporter gene ( Zat12::luciferase ), and a probe specific to hydrogen peroxide (HyPer), we demonstrated that this aphid induces accumulation of reactive oxygen species (ROS) in Arabidopsis thaliana . Similar to the apoplastic oxidative burst induced by pathogens, this response to aphids was rapid and transient, with two peaks occurring within 1 and 4 hr after infestation. Aphid infestation also induced an oxidative response in the cytosol and peroxisomes, as measured using a redox‐sensitive variant of green fluorescent protein (roGFP2). This intracellular response began within minutes of infestation but persisted 20 hr or more after inoculation, and the response of the peroxisomes appeared stronger than the response in the cytosol. Our results suggest that the oxidative response to aphids involves both apoplastic and intracellular sources of ROS, including ROS generation in the peroxisomes, and these different sources of ROS may potentially differ in their impacts on host suitability for aphids.