English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Summary   Streptomyces scabies  causes potato common scab disease, which reduces the quality and market value of affected tubers. The predominant pathogenicity determinant produced by  S. scabies  is the thaxtomin A phytotoxin, which is essential for common scab disease development. Production of thaxtomin A involves the nonribosomal peptide synthetases (NRPSs) TxtA and TxtB, both of which contain an adenylation (A‐) domain for selecting and activating the appropriate amino acid during thaxtomin biosynthesis. The genome of  S. scabies  87.22 contains three small MbtH‐like protein (MLP)‐coding genes, one of which ( txtH ) is present in the thaxtomin biosynthesis gene cluster. MLP family members are typically required for the proper folding of NRPS A‐domains and/or stimulating their activities. This study investigated the importance of TxtH during thaxtomin biosynthesis in  S. scabies . Biochemical studies showed that TxtH is required for promoting the soluble expression of both the TxtA and TxtB A‐domains in  Escherichia coli , and amino acid residues essential for this activity were identified. Deletion of  txtH  in  S. scabies  significantly reduced thaxtomin A production, and deletion of one of the two additional MLP homologues in  S. scabies  completely abolished production. Engineered expression of all three  S. scabies  MLPs could restore thaxtomin A production in a triple MLP‐deficient strain, while engineered expression of MLPs from other  Streptomyces  spp. could not. Furthermore, the constructed MLP mutants were reduced in virulence compared to wild‐type  S. scabies.  The results of our study confirm that TxtH plays a key role in thaxtomin A biosynthesis and plant pathogenicity in  S. scabies.

TxtH is a key component of the thaxtomin biosynthetic machinery in the potato common scab pathogen Streptomyces scabies