English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Streptococcus sanguinis  is an important cause of infective endocarditis. In strain SK36, the ABC‐family manganese transporter, SsaACB, is essential for virulence. We have now identified a ZIP‐family protein, TmpA, as a secondary manganese transporter. A  tmpA  mutant had no phenotype, but a Δ ssaACB  Δ tmpA  mutant was more attenuated for serum growth and for virulence in a rabbit model than its Δ ssaACB  parent. The growth of both mutants was restored by supplemental manganese, but the Δ ssaACB  Δ tmpA  mutant required twenty‐fold more and accumulated less. Although ZIP‐family proteins are known for zinc and iron transport, TmpA‐mediated transport of either metal was minimal. While  ssaACB  appears ubiquitous in  St. sanguinis ,  tmpA  was present in a majority of strains and a  mntH  gene encoding an NRAMP‐family transporter was identified in relatively few, including VMC66. As in SK36, deletion of  ssaACB  greatly diminished VMC66 endocarditis virulence and serum growth, and deletion of  tmpA  from this mutant diminished virulence further. Virulence was not significantly altered by deletion of  mntH  from either VMC66 or its Δ ssaACB  mutant. This and the accompanying paper together suggest that SsaACB is of primary importance for endocarditis virulence while secondary transporters TmpA and MntH contribute to growth under differing conditions.

Contribution of a ZIP‐family protein to manganese uptake and infective endocarditis virulence in Streptococcus sanguinis