Open AccessCorrelation of plasma coagulation tests and fibrinogen Clauss with rotational thromboelastometry parameters and prediction of bleeding in dogs
Author(s) -
Enk Nathalie M.,
Kutter Annette P. N.,
KuemmerleFraune Claudia,
Sigrist Nadja E.
Publication year - 2018
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.15365
Subject(s) - thromboelastometry , fibrinogen , medicine , prothrombin time , clotting time , partial thromboplastin time , coagulation , coagulation testing , anesthesia , platelet , cardiology
Background Correlation of plasma fibrinogen concentration (fibrinogen Clauss ) with rotational thromboelastometry (ROTEM) parameters has not been investigated in dogs. Objectives To determine the correlation between plasma coagulation tests and fibrinogen Clauss with ROTEM parameters and to evaluate their ability to predict bleeding in dogs. Animals Ninety‐seven dogs with concurrent determination of fibrinogen Clauss and fibrin polymerization test (FIBTEM) analysis. Methods Signalment, pretreatment, clinical signs of bleeding, fibrinogen Clauss , plasma coagulation test results, hematocrit, platelet count, FIBTEM, extrinsic (EXTEM) and intrinsic (INTEM) activated ROTEM assays were retrieved retrospectively. Correlations between fibrinogen Clauss and FIBTEM maximum clot firmness (MCF FIBTEM ) and between prothrombin time (PT) or activated partial thromboplastin time (aPTT) and ROTEM parameters were determined. Dogs were further assigned to groups with or without clinical signs of bleeding. The prognostic significance of significantly different parameters to predict bleeding was evaluated. Results Fibrinogen Clauss showed strong correlation with MCF FIBTEM (r = 0.860, n = 97, P < .001). PT showed strong correlation with EXTEM clotting time (CT EXTEM ) (r = 0.839, n = 53, P < .001), and aPTT was strongly correlated with INTEM CT (CT INTEM ) (r = 0.664, n = 31, P < .001). Platelet count, PT/aPTT, EXTEM clot formation time (CFT EXTEM ), MCF EXTEM , EXTEM maximum clot elasticity (MCE EXTEM ), and CT INTEM were significantly different between groups. A CT INTEM >149 seconds was 100% sensitive to detect bleeding. Conclusions and Clinical Importance The MCF FIBTEM can be used to evaluate the effect of fibrinogen on hemostasis as an alternative to determination of fibrinogen Clauss . In addition, CT EXTEM and CT INTEM are strongly correlated with PT and aPTT, respectively.