Open AccessKinetics of Plasma Cell‐Free DNA and Creatine Kinase in a Canine Model of Tissue Injury
Author(s) -
Wilson I.J.,
Burchell R.K.,
Worth A.J.,
Burton S.E.,
Gedye K.R.,
Clark K.J.,
Crosse K.R.,
Jack M.,
Odom T.F.,
De Grey S.J.,
McGlade K.M.S.,
Tomlin S.C.,
LopezVillalobos N.,
Gal A.
Publication year - 2017
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.14901
Subject(s) - medicine , creatine kinase , cruciate ligament , cell free fetal dna , surgery , anesthesia , gastroenterology , anterior cruciate ligament , fetus , pregnancy , prenatal diagnosis , biology , genetics
Background Cell‐free DNA (cf DNA ) comprises short, double‐stranded circulating DNA sequences released from damaged cells. In people, cf DNA concentrations correlate well with disease severity and tissue damage. No reports are available regarding cf DNA kinetics in dogs. Objectives/Hypothesis Cell‐free DNA will have a short biological half‐life and would be able to stratify mild, moderate, and severe tissue injury. Our study aims were to determine the kinetics and biological half‐life of cf DNA and to contrast them with those of creatine kinase ( CK ). Animals Three groups of 10 dogs undergoing open ovariohysterectomy, surgery for cranial cruciate ligament rupture ( CCLR ), or hemilaminectomy. Methods Plasma for cf DNA and CK analysis was collected at admission, at induction of anesthesia, postsurgery (time 0) and at 6, 12, 24, 36, 48, 60, and 72 hours after surgery. Results The biological half‐life of plasma cf DNA and CK were 5.64 hours (95% confidence interval [ CI 95], 4.36–7.98 hours) and 28.7 hours ( CI 95, 25.3–33.3 hours), respectively. In the hemilaminectomy group, cf DNA concentrations differed significantly from admission at 6–12 hours after surgery. Creatine kinase activity differed among the surgical groups and reached a peak 6 hours after surgery. In the ovariohysterectomy and CCLR groups, plasma CK activity 72 hours after surgery did not differ from admission activity of the ovariohysterectomy group. In contrast, in the hemilaminectomy group, plasma CK activity after 72 hours did not return to the ovariohysterectomy group admission activity. Conclusions and Clinical Importance Plasma CK activity has a longer biological half‐life than previously thought. In contrast to plasma CK activity, cf DNA has a short half‐life and could be a useful marker for peracute severe tissue injury.