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Background  Antiplatelet medications are increasingly used in dogs. Remote analysis of platelet activity is challenging, limiting assessment of antiplatelet drug efficacy.    Hypothesis/Objectives  To evaluate a method used in humans for stimulation and remote analysis of canine platelet activity.    Animals  Forty‐five dogs of various ages without a coagulopathy or thrombocytopenia. Six were receiving antiplatelet medication.    Methods  Prospective observational study. Platelets were stimulated with combinations of arachidonic acid ( AA ) and epinephrine (Epi) or adenosine diphosphate ( ADP ) and the thromboxane A 2 ‐mimetic U46619 (U4).  PAMF ix was added to the blood samples to facilitate delayed analysis of platelet activity. Activity was assessed by flow cytometric measurement of surface P‐selectin ( CD 62P) expression.    Results  Canine platelets could be stimulated with both  AA /Epi and  ADP /U4. The levels of P‐selectin were significantly greater than paired, unstimulated samples ( P  < 0.001). Inhibition of P‐selectin expression occurred after this stimulation by adding antiplatelet drugs in vitro. The efficacy of antiplatelet drugs in samples from treated dogs was also measurable ex vivo using this method. Delayed analysis of platelet activity at time points up to 22 days demonstrated excellent correlation between respective mf values at each time point (r 2  = 0.92,  P  < 0.0001).    Conclusions and Clinical Importance  This study evaluated a new method to remotely assess canine platelet activity. It shows that  PAMF ix can be used for this purpose. This provides opportunities to interrogate the inhibitory action of antiplatelet drugs in clinical settings.

A Remote Assay for Measuring Canine Platelet Activation and the Inhibitory Effects of Antiplatelet Agents