Repeatability of Oral Sugar Test Results, Glucagon‐Like Peptide‐1 Measurements, and Serum High‐Molecular‐Weight Adiponectin Concentrations in Horses

Background Repeatability of the oral sugar test ( OST ) has not been evaluated. Objectives We hypothesized that OST glucose, insulin, active ( aGLP ‐1) and total ( tGLP ‐1) glucagon‐like peptide 1, and high‐molecular‐weight ( HMW ) adiponectin results would be repeatable. Animals Fifty‐three horses from a Tennessee research facility (n = 23) and private practice in Missouri (n = 30), including animals with medical histories of equine metabolic syndrome. Methods Two OST s were performed 7–14 days apart and plasma glucose and insulin concentrations were measured at 0, 60, and 75 minutes; a positive result was defined as detection of an insulin concentration >45 μU/mL at 60 or 75 minutes. Plasma aGLP ‐1 and serum tGLP ‐1 concentrations at 75 minutes and serum HMW adiponectin concentrations at 0 minute were measured in the Missouri group. Bland‐Altman analyses were performed. Results No adverse events were reported. Bland‐Altman analysis indicated mean ±  SD bias of 1.5 ± 14.8 μU/mL (95% confidence interval [ CI ], −27.6 to 30.5 μU/mL) and 1.2 ± 16.1 μU/mL (95% CI , −30.4 to 32.8 μU/mL) for insulin concentrations at 60 and 75 minutes, respectively. There was 91 and 83% agreement in test interpretation between test days for OST insulin results for all horses in the Tennessee and Missouri groups, respectively. Conclusions and Clinical Importance Repeatability of the OST was acceptable when values obtained from Bland‐Altman analyses were evaluated, and there was good agreement in binary (negative/positive) test interpretation for insulin concentrations. However, wide 95% CI s were detected for insulin concentrations.