Open AccessCommercially Available Enzyme‐Linked Immunosorbent Assay and Polymerase Chain Reaction Tests for Detection of Feline Immunodeficiency Virus Infection
Author(s) -
Nichols J.,
Weng H.Y.,
Litster A.,
Leutenegger C.,
Guptill L.
Publication year - 2016
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.14579
Subject(s) - medicine , cats , polymerase chain reaction , pcr test , kappa , feline immunodeficiency virus , virology , real time polymerase chain reaction , human immunodeficiency virus (hiv) , viral disease , lentivirus , biology , biochemistry , linguistics , philosophy , gene
Background Feline immunodeficiency virus (FIV) infection is an important cause of disease of cats worldwide. Initial screening is commonly performed by commercially available point‐of‐care (POC) ELISA tests. Confirmatory testing for positive POC test results is recommended. Polymerase chain reaction (PCR) tests for FIV are commonly used additional testing methods; however, reported measures of diagnostic accuracy vary widely between PCR tests, making interpretation of results difficult. Hypothesis/Objective There is very good agreement between results of a commercially available PCR test and a POC ELISA test for FIV for specimens collected from owned and shelter‐housed cats. Animals Blood samples from 168 cats from 2 adoption guarantee shelters, an FIV Sanctuary, and 64 private homes were used. Methods This was a prospective study. Whole blood samples were collected in K 2 ‐EDTA, divided, and submitted for PCR and ELISA testing. Follow‐up whole blood samples were collected in lithium heparin from cats with discordant results and submitted for virus isolation (VI). Results There was very good agreement between ELISA and PCR (kappa 0.87; P < .001; 95% CI 0.79, 0.95). Of 168 cats, eleven had discordant ELISA/PCR results: 7 ELISA+/PCR‐ and 4 ELISA‐/PCR+. Using VI as a reference standard, there were 4 false‐positive PCR results, 5 false‐positive ELISA results, and 1 false‐negative PCR result (1 cat lost to follow‐up). Conclusions and Clinical Importance While there was good agreement between the POC ELISA and PCR tests, the discordant results highlight the importance of cautious interpretation of test results and the necessity of confirmatory testing .