Open AccessSerum Surfactant Protein D and Haptoglobin as Potential Biomarkers for Inflammatory Airway Disease in Horses
Author(s) -
Bullone M.,
Lagarde M.,
Vargas A.,
Lavoie J.P.
Publication year - 2015
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.13602
Subject(s) - haptoglobin , medicine , serum amyloid a , inflammation , acute phase protein , immunology , c reactive protein , surfactant protein d , horse , gastroenterology , immune system , innate immune system , biology , paleontology
Background The identification of serum biomarkers of lung inflammation would facilitate the diagnosis of inflammatory airway disease ( IAD ) in horses. Hypothesis Horses with IAD have higher serum concentrations of markers of inflammation compared to controls. Animals Twelve horses with IAD and 10 control horses. Methods This was a prospective case–control study. Blood and BALF were collected from horses with IAD and controls. Serum concentration of surfactant protein D ( SP ‐D), haptoglobin, serum amyloid A ( SAA ) and of the soluble form of triggering receptor expressed on myeloid cells 1 ( sTREM ‐1) was measured using commercial ELISA tests. Results Horses with IAD had higher serum concentration (log‐transformed values) of SP ‐D (mean ± SD : 1.773 ± 0.51), haptoglobin (6.657 ± 0.202) and SAA (0.128 ± 0.396) compared to controls (0.942 ± 0.226, 6.38 ± 0.22, −0.398 ± 0.319, respectively; P < .01 for all). Furthermore, the concentrations of SP ‐D and haptoglobin combined allowed differentiating the 2 groups ( IAD : 8.43 ± 0.564, controls: 7.322 ± 0.249, P < .0001) with a sensitivity and specificity of 100% when a cut‐off of 7.70 (log value) was employed. Conclusions and Clinical Importance Surfactant protein D and haptoglobin serum concentrations could be a diagnostic aid in IAD . Further studies are necessary to establish the specificity of our findings before they can be applied in everyday practice.