Open AccessSerial Evaluation of Abdominal Fluid and Serum Amino‐terminal pro‐C‐type Natriuretic Peptide in Dogs with Septic Peritonitis
Author(s) -
Guieu L.V.S.,
Bersenas A.M.E.,
Holowaychuk M.K.,
Brisson B.A.,
Weese J.S.
Publication year - 2015
Publication title -
journal of veterinary internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.356
H-Index - 103
eISSN - 1939-1676
pISSN - 0891-6640
DOI - 10.1111/jvim.13575
Subject(s) - medicine , abdominal cavity , peritonitis , abdominal fluid , gastroenterology , surgery
Background Serum N ‐terminal pro‐ C ‐natriuretic peptide ( NT ‐pro CNP ) has shown promise as a diagnostic biomarker for sepsis. Its sensitivity to detect dogs with septic peritonitis ( SP ) is reportedly low, perhaps attributable to the compartmentalization of NT ‐pro CNP in the abdominal cavity. Objectives To evaluate the use of an ELISA for the measurement of NT ‐pro CNP in canine abdominal fluid and to describe the peri‐operative pattern of abdominal fluid and serum NT ‐pro CNP concentrations in dogs with SP . Animals Five client‐owned dogs with nonseptic abdominal effusion of varying etiologies and 12 client‐owned dogs with SP undergoing abdominal surgery and placement of a closed‐suction abdominal drain ( CSAD ). Six dogs were included upon hospital admission; 6 were included the day after surgery. Methods Prospective pilot study. A commercially available ELISA kit was analytically validated for use on canine abdominal fluid. The NT ‐pro CNP concentrations were measured in the abdominal fluid of control dogs, and in serum and abdominal fluid of dogs with SP from admission for CSAD removal. Results In dogs with SP , admission abdominal fluid NT ‐pro CNP concentrations were lower than the concurrent serum concentrations ( P = 0.031), and lower than control canine abdominal fluid concentrations ( P = 0.015). Postoperatively, abdominal fluid NT ‐pro CNP concentrations remained lower than serum concentrations ( P < 0.050), except on day 4. Conclusions and Clinical Importance The ELISA kit was able to measure NT ‐pro CNP in canine abdominal fluid. In dogs with SP , low serum NT ‐pro CNP concentrations cannot be explained by abdominal compartmentalization.