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Ligand‐Based Molecular MRI: O‐17 JJVCPE Amyloid Imaging in Transgenic Mice
Author(s) -
Suzuki Kiyotaka,
Igarashi Hironaka,
Huber Vincent J.,
Kitaura Hiroki,
Kwee Ingrid L.,
Nakada Tsutomu
Publication year - 2014
Publication title -
journal of neuroimaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.822
H-Index - 64
eISSN - 1552-6569
pISSN - 1051-2284
DOI - 10.1111/jon.12091
Subject(s) - molecular imaging , in vivo , medicine , molecular probe , ligand (biochemistry) , nuclear magnetic resonance , preclinical imaging , gadolinium , magnetic resonance imaging , mri contrast agent , nuclear medicine , chemistry , radiology , receptor , biochemistry , biology , dna , physics , microbiology and biotechnology , organic chemistry
BACKGROUND Development of molecular MR imaging (MRI) similar to PET imaging using contrast agents such as gadolinium as probe have been inherently hampered by incompatibility between potential probe (charged molecules) and membrane permeability. Nevertheless, considering the inherent spatial resolution limit for PET of 700μ, the superior microscopic resolution of MRI of 4 μ presents a strong incentive for research into ligand‐based molecular MRI. METHODS 17 O exhibits JJ vicinal coupling with a covalently bound proton in a hydroxyl group. This 17 O coupled proton can be ionized in water solution and interexchange with other water protons. This property can be utilized as “probe” in T2‐weighted imaging and developed into ligand‐based molecular MRI. We examined β‐amyloid distribution in human APP overexpressed transgenic mice in vivo following injection of 17 O labeled Pittsburg compound B ( 17 O‐PiB). RESULTS JJVCPE imaging successfully imaged 17 O‐PiB, unequivocally establishing that 17 O JJVCPE imaging can be developed into PET‐like molecular MRI in clinical medicine. CONCLUSIONS The study represents the first successful ligand‐based molecular MRI in vivo . This is also the first in vivo amyloid imaging using MRI. High‐resolution molecular MRI with high specificity under clinical settings, such as in vivo microscopic imaging of senile plaque, is a foreseeable aim.