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Interleukin‐6 regulates expression of Fos and Jun genes to affect the development of mouse preimplantation embryos
Author(s) -
Yu Chunhua,
Zhang Xinyan,
Wang Li,
Liu Yinan,
Li Na,
Li Min,
Chen Li,
Liu Yingyu,
Yao Yuanqing
Publication year - 2018
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.13498
Subject(s) - blastocyst , embryo , andrology , gene expression , messenger rna , biology , embryogenesis , microbiology and biotechnology , medicine , endocrinology , gene , genetics
Aim We investigated whether recombinant mouse interleukin‐6 ( IL ‐6) affects the development of preimplantation embryos and induces the ‐ s ignal transducers and activators of transcription ( JAK – STAT ) signaling pathway by binding IL ‐6 signal transducer ( IL ‐6st) and regulating Fos and Jun gene expression, thereby accounting for the negative effect of superovulation on embryo development. Methods We compared rates of blastocyst formation from embryos cultured with different concentrations of IL ‐6 or/and anti‐interleukin 6 receptor antibody (anti‐ IL ‐6 RA b) in superovulated experimental and normal control groups. IL ‐6 expression in preimplantation embryos was determined by immunofluorescence identification. F os, J un and IL ‐6st messenger RNA expression was detected by PCR and microarray experiments. Results Rates of blastocyst formation significantly decreased in superovulated embryos, whether or not they were incubated in 0.1, 1, 25 or 50 pg/m L IL ‐6, ( P < 0.01) compared to embryos from naturally ovulated controls, whereas incubation in 5 and 10 pg/m L IL ‐6 reversed the negative effects of superovulation. The addition of anti‐ IL ‐6 RA b to naturally ovulated embryos reduced blastocyst rates to those of superovulated embryos. Gene chip analysis indicated that the JAK – STAT signaling pathway contained differentially expressed IL ‐6 , IL ‐6st , Jun and Fos genes. Both anti‐ IL ‐6 RA b or ovarian stimulation downregulated IL ‐6st, J un, and F os messenger RNA expression, but expression of the same three genes increased in 5 pg/m L IL ‐6. Conclusion Ovarian stimulation negatively impacts the development of preimplantation embryos by reducing IL ‐6 release. IL ‐6 affects the rate of development of zygotes to blastocyst by regulating IL ‐6 st, F os and J un expression in the JAK – STAT signaling pathway.