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Development and utilization of a new chemically‐induced soybean library with a high mutation density
Author(s) -
Li Zhongfeng,
Jiang Lingxue,
Ma Yansong,
Wei Zhongyan,
Hong Huilong,
Liu Zhangxiong,
Lei Jinhui,
Liu Ying,
Guan Rongxia,
Guo Yong,
Jin Longguo,
Zhang Lijuan,
Li Yinghui,
Ren Yulong,
He Wei,
Liu Ming,
Htwe Nang Myint Phyu Sin,
Liu Lin,
Guo Bingfu,
Song Jian,
Tan Bing,
Liu Guifeng,
Li Maiquan,
Zhang Xianli,
Liu Bo,
Shi Xuehui,
Han Sining,
Hua Sunan,
Zhou Fulai,
Yu Lili,
Li Yanfei,
Wang Shuang,
Wang Jun,
Chang Ruzhen,
Qiu Lijuan
Publication year - 2017
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12505
Subject(s) - biology , mutant , ethyl methanesulfonate , genetics , allele , population , phenotype , gene , single nucleotide polymorphism , mutation breeding , mutation , genotype , demography , sociology
Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate (EMS)‐induced soybean ( Glycine max ) population, consisting of 21,600 independent M 2 lines, was developed. Over 1,000 M 4 (5) families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, g yl, that displayed significantly decreased chlorophyll (Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to MinnGold, where a different single nucleotide polymorphism variation in the Mg‐chelatase subunit gene ( ChlI1a ) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker‐assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.