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Method for cryopreservation of Paramoeba perurans
Author(s) -
Tröße Christiane,
Kindt Mats,
Blindheim Steffen,
Andersen Linda,
Nylund Are
Publication year - 2021
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.13295
Subject(s) - cryopreservation , biology , vial , parasite hosting , bioassay , in vitro , andrology , genetics , embryo , chemistry , medicine , chromatography , world wide web , computer science
Paramoeba perurans causes amoebic gill disease (AGD), which is a major problem in aquaculture worldwide. The parasite can be cultured in vitro , but to this date, no method for long‐term storage of the clones exists. In this study, we describe a method for cryopreservation of Paramoeba perurans . The method was successfully employed on four out the five clones we tested. The thawing success rate, that is the percentage of successfully thawed vials relative to the total number of vials that were thawed, differed for the clones and ranged from 25% to 100%. The age of the clones seemed to have a negative impact on the ability to survive cryopreservation.