Open AccessSilent hemoglobin variant during capillary electrophoresis: A case report
Author(s) -
Yuan Yanping,
Zhou Xianghai,
Gao Leili,
Ren Qian,
Ji Lig
Publication year - 2020
Publication title -
journal of diabetes investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.089
H-Index - 50
eISSN - 2040-1124
pISSN - 2040-1116
DOI - 10.1111/jdi.13222
Subject(s) - capillary electrophoresis , medicine , glycated hemoglobin , hemoglobin variants , sanger sequencing , hemoglobin , ion chromatography , diabetes mellitus , chromatography , glycated haemoglobin , gene , type 2 diabetes , dna sequencing , genetics , endocrinology , biology , chemistry
Abstract Hemoglobin (Hb) North Manchester [β51(D2) Pro → His; HBB :c.155 C>A] is a rare Hb β‐globin gene variant that affects glycated Hb measurement values, such as ion‐exchange high‐performance liquid chromatography, in patients with diabetes. This variant was first detected in the UK in 1998. Here, we describe the first case involving Hb North Manchester detected incidentally in a patient with type 2 diabetes in Northern China. The Hb variant was discovered by ion‐exchange high‐performance liquid chromatography, yet capillary electrophoresis of both glycated Hb program and Hb program failed to detect it. Subsequently, Sanger sequencing was carried out to help identify the Hb variant.