Open AccessDetailed analysis at a single‐cell level of cells undergoing pancreatic differentiation
Author(s) -
Shiraki Nobuaki,
Kume Shoen
Publication year - 2020
Publication title -
journal of diabetes investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.089
H-Index - 50
eISSN - 2040-1124
pISSN - 2040-1116
DOI - 10.1111/jdi.13140
Subject(s) - enteroendocrine cell , cell , microbiology and biotechnology , cell sorting , stem cell , cellular differentiation , mesenchymal stem cell , induced pluripotent stem cell , medicine , endocrine system , biology , hormone , biochemistry , embryonic stem cell , gene
Pancreatic cells derived from pluripotent stem cells using the stem cell‐derived‐β (SC‐β) differentiation protocol are composed of four main cell types, namely β‐like cells (SC‐β‐cells), α‐like cells, enterochromaffin‐like cells and non‐endocrine cells. Single‐cell dissociation and reaggregation depleted non‐endocrine cells and improved β‐cell function. Veres et al . succeeded in efficiently purifying SC‐β by magnetic cell sorting using CD49a antibody and reaggregation, so that the final SC‐β‐cell ratio increased to 80%.