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Aims/Introduction  Endothelial cell inflammatory injury is likely required for barrier dysfunction under hyperglycemic conditions. Curcumin ( CUR ) is well known for its anti‐inflammatory effect. However, there have been few reports about the anti‐inflammatory effect of  CUR  induced by high glucose in endothelial cells. The aim of the present study was to investigate the inflammatory effect of high glucose and the anti‐inflammatory effect of  CUR  induced by high glucose in rat thoracic aorta endothelial cells ( TAEC s).    Materials and Methods  Well characterized  TAEC s were established and cell viability was assayed by the cell counting kit‐8 method, messenger ribonucleic acid and protein expression were identified by real‐time polymerase chain reaction, western blot or enzyme‐linked immunosorbent assay, respectively. The production of reactive oxygen species was observed by a fluorescence microscope.    Results  High glucose (30 mmol/L) significantly decreased the cell viability of  TAEC s after being co‐cultivated for 12 h and showed a time‐dependent manner, and increased interleukin ( IL )‐1β,  IL ‐6 and tumor necrosis factor‐α secretion in  TAEC s. The injury effect of high glucose was involved in the reactive oxygen species–phosphoinositide 3‐kinase ( PI 3K)/protein kinase B ( AKT )–nuclear factor ( NF )‐κB signaling pathway. Anti‐oxidant N‐acetylcysteine,  PI 3K and  NF ‐κB‐specific pathway inhibitors can abolish the secretion of these inflammatory factors; pretreatment with anti‐oxidant N‐acetylcysteine significantly decreased  PI 3K expression, the level of phosphorylated  AKT  and nuclear  NF ‐κB; pretreatment of  LY 294002 can significantly decrease the  NF ‐κB level in nuclei. After treatment with  CUR  for 12 h,  IL ‐1β,  IL ‐6 and tumor necrosis factor‐α secretion were markedly decreased, and  PI 3K expression, the phosphorylation of  AKT  and nuclear  NF ‐κB level were also decreased.    Conclusion  Curcumin attenuates high glucose‐induced inflammatory injury through the reactive oxygen species– PI 3K/ AKT – NF ‐κB signaling pathway in rat thoracic aorta endothelial cells.

journal of diabetes investigation

Curcumin attenuates high glucose‐induced inflammatory injury through the reactive oxygen species–phosphoinositide 3‐kinase/protein kinase B–nuclear factor‐κB signaling pathway in rat thoracic aorta endothelial cells