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Aim  To determine the impact of the Best Practice Spotlight Organization® initiative on nurses’ perception of their work environment and their attitudes to evidence‐based practice.    Design  Quasi‐experimental, multicentre study. The intervention is the participation in Best Prectice Spotilight Organizations to implement Best Practice Guidelines.    Methods  The study will include seven centres in the interventional group and 10 in the non‐equivalent control group, all of them belonging to the Spanish national health system. The Practice Environment Scale of the Nursing Work Index, and the Health Sciences Evidence‐Based Practice Questionnaire will be administered to a sample of 1,572 nurses at the beginning of the programme and at 1 year. This 3‐year study started in April 2018 and will continue until December 2021. Statistical analyses will be carried out using the SPSS 25.0. This project was approved by the Drug Research Ethics Committee of the Parc de Salut Mar and registered in Clinical Trials.    Discussion  The study findings will show the current state of nurses’ perception of their work environment and attitudes to evidence‐based practice, and possible changes in these parameters due to the programme.    Impact  The findings could provide a strong argument for health policymakers to scale up the Best Practice Spotlight Organization® initiative in the Spanish national health system.

实施最佳实践指南对护士循证实践和护士工作环境的影响:研究方案