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Aim  Evaluation of the thermal and physical conditions for inactivation of adenovirus (AdV), porcine sapelovirus 1 ( PSV 1) and the economically important viruses porcine epidemic diarrhoea virus ( PEDV ) and porcine circovirus 2 ( PCV 2) in the production of spray‐dried porcine plasma ( SDPP ).    Methods and Results  Citrate‐treated porcine plasma of  pH  7·5, 9·8 and 10·2 (8·5% dry‐matter) was spiked with  PEDV ,  PSV 1,  PCV 2 and AdV and incubated at 3°C for maximum 24 h, and at 44 or 48°C for maximum 10 min (Experiment 1). Spiked citrate‐treated concentrated plasma of  pH  7·5 and 9·8 (24% dry‐matter) was spray dried in a laboratory scale apparatus (Experiment 2). Aliquots of  SDPP  were stored over a period of 0–10 weeks at 11 and 20°C (Experiment 3). Reverse transcription(RT)‐quantitative PCR detected no notable reduction in viral genomes in treated plasma and  SDPP  samples. No infectious  PSV 1 was re‐isolated from plasma and  SDPP  samples in cell culture. At  pH  10·2 and 3°C, infectivity of  PEDV  in plasma was reduced with a reduction factor of 4·2 log 10 ( LRF ) at 10 h contact time, whereas heating to 44°C for at least 1 min at alkali  pH  was needed to achieve a  LRF  of 4·2 for AdV. Spray drying at an outlet temperature of 80°C reduced AdV infectivity effectively ( LRF  = 5·2) and  PEDV  infectivity for 95% ( LRF  = 1·4). After storage at 20°C for 2 weeks no infectious  PEDV  was re‐isolated from  SDPP  anymore ( LRF  ≥4·0). Due to growth of antibiotic‐resistant bacteria from plasma in cell cultures used for  PCV 2 isolation, no data regarding inactivation of  PCV 2 were obtained.    Conclusions  Five percent of  PEDV  stayed infectious after our spray drying conditions. Spray drying in combination with storage for ≥2 weeks at 20°C eliminated infectivity of  PEDV  effectively.    Significance and Impact of the Study  The conditions for inactivation of virus in plasma and  SDPP  determined are important for producers to inactivate  PEDV  during production of  SDPP .

journal of applied microbiology

Study on inactivation of porcine epidemic diarrhoea virus, porcine sapelovirus 1 and adenovirus in the production and storage of laboratory spray‐dried porcine plasma