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Biological tissue as a active substances drug prolonged diffusion support
Author(s) -
Grossetete Florine,
Barnouin Laurence
Publication year - 2019
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2019.5484
Subject(s) - titration , ground substance , chemistry , umbilical cord , desiccation , diffusion , elastin , biomedical engineering , freeze drying , chromatography , surgery , connective tissue , medicine , pathology , anatomy , biology , organic chemistry , botany , physics , thermodynamics
Objective Use of ground proceed umbilical cord lining (Wharton Jelly) for an prolonged diffusion of active substance with a progressive disintegration of the tissue within few days. Methods Ground proceed umbilical cord lining is a tissue that contains proteoglycans, TGFß, elastin, and collagen. By calibrating the grinding and associating a freeze‐drying with a second desiccation at 35°C‐ 50 microBar, fibers are sticking together and enable having a compact and shaped final product. The addition of solution of gentamycin sulfate in the ground gelatinous tissue is performed before the step of freeze‐drying.Titrations of gentamycin in the solution of ground tissues before and after freeze‐drying are performed according to the method A from European Pharmacopeia. Results Titration of gentamycin mixed with ground tissues is from an average of 3024 UI/sample (SD 94) before freeze‐drying. After freeze‐drying: 2997 UI/sample (SD:189). This titration is the one expected regarding the solution preparation, so there is no loss of active principle activity. Titration in time submitted to mechanical and hydration stress are in progress. Discussion Tissues extracted from Wharton Jelly are products used for different pathologies of ocular surface. The use of a solid form, shaped at convenience can enable diffusion of active substance drug to treat more complex infections of the ocular surface and also in eye chambers.