Premium
Sexing European rabbits ( Oryctolagus cuniculus ), European brown hares ( Lepus europaeus ) and mountain hares ( Lepus timidus ) with ZFX and ZFY loci
Author(s) -
FONTANESI LUCA,
TAZZOLI MARCO,
PECCHIOLI ELENA,
HAUFFE HEIDI C.,
ROBINSON TERENCE J.,
RUSSO VINCENZO
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02167.x
Subject(s) - biology , sexing , european rabbit , zoology , population , restriction fragment length polymorphism , polymerase chain reaction , genetics , gene , demography , sociology
We describe a new method for the sex determination of tissue originating from Oryctolagus cuniculus (European rabbit), Lepus europaeus (European brown hare) and Lepus timidus (mountain hare) based on PCR‐RFLP analysis of point mutations that differentiate the ZFX and ZFY gene sequences. Among several applications, this PCR‐RFLP method could be used to investigate gender ratio and evaluate the population dynamics of these species using samples collected when sex cannot be identified.