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Microscopic Study of Crystal Growth in Cryopreservation Agent Solutions and Water
Author(s) -
TAO LEREN,
HUA TSECHAO
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04566.x
Subject(s) - electric field , distilled water , ice crystals , cryopreservation , voltage , ionic bonding , materials science , crystal (programming language) , chemistry , analytical chemistry (journal) , chemical engineering , chromatography , optics , ion , physics , biology , organic chemistry , embryo , engineering , quantum mechanics , computer science , programming language , microbiology and biotechnology
A bstract : Ice formation inside or outside cells during cryopreservation is evidently the main factor of cryoinjury to cells. In the study described here a high voltage DC electric field and a cryomicroscopic stage were used to test DMSO and NaCl solutions under electric field strengths ranging from 83 kV/m to 320 kV/m. Dendritic ice crystals became asymmetric when the electric field was activated. This change in the ice crystal shape was more pronounced in the ionic NaCl solution. In addition, ice growth of distilled water without an electric field was tested under different cooling rates.