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Caveolin‐1 influences P2X 7 receptor expression and localization in mouse lung alveolar epithelial cells
Author(s) -
Barth K.,
Weinhold K.,
Guenther A.,
Young M. T.,
Schnittler H.,
Kasper M.
Publication year - 2007
Publication title -
the febs journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 204
eISSN - 1742-4658
pISSN - 1742-464X
DOI - 10.1111/j.1742-4658.2007.05830.x
Subject(s) - caveolin 1 , caveolae , colocalization , lipid raft , caveolin , microbiology and biotechnology , lung , biology , receptor , signal transduction , medicine , biochemistry
The P2X 7 receptor has recently been described as a marker for lung alveolar epithelial type I cells. Here, we demonstrate both the expression of P2X 7 protein and its partition into lipid rafts in the mouse lung alveolar epithelial cell line E10. A significant degree of colocalization was observed between P2X 7 and the raft marker protein Caveolin‐1; also, P2X 7 protein was associated with caveolae. A marked reduction in P2X 7 immunoreactivity was observed in lung sections prepared from Caveolin‐1‐knockout mice, indicating that Caveolin‐1 expression was required for full expression of P2X 7 protein. Indeed, suppression of Caveolin‐1 protein expression in E10 cells using short hairpin RNAs resulted in a large reduction in P2X 7 protein expression. Our data demonstrate a potential interaction between P2X 7 protein and Caveolin‐1 in lipid rafts, and provide a basis for further functional and biochemical studies to probe the physiologic significance of this interaction.