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Oxidized low‐density lipoprotein increases interleukin‐8 production in human gingival epithelial cell line Ca9‐22
Author(s) -
Suzuki K.,
Sakiyama Y.,
Usui M.,
Obama T.,
Kato R.,
Itabe H.,
Yamamoto M.
Publication year - 2010
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2009.01263.x
Subject(s) - scavenger receptor , chemistry , prostaglandin e2 , inflammation , interleukin , cell culture , interleukin 8 , lipoprotein , microbiology and biotechnology , endocrinology , medicine , cytokine , immunology , biochemistry , biology , cholesterol , genetics
Suzuki K, Sakiyama Y, Usui M, Obama T, Kato R, Itabe H, Yamamoto M. Oxidized low‐density lipoprotein increases interleukin‐8 production in human gingival epithelial cell line Ca9‐22. J Periodont Res 2010; 45: 488–495. © 2010 John Wiley & Sons A/SBackground and Objective: Recent epidemiological studies have shown a correlation between periodontitis and hyperlipidemia. We have found high levels of oxidized low‐density lipoprotein (OxLDL) in the gingival crevicular fluid of dental patients. In the present study, we tried to examine the possible role of OxLDL in periodontal inflammation in vitro . Material and Methods: Cells of the human gingival epithelial cell line Ca9‐22 were cultured in media containing OxLDL, and the amounts of interleukin‐8 (IL‐8) and prostaglandin E 2 (PGE 2 ) produced were measured using ELISAs. Results: Production of IL‐8 by Ca9‐22 cells was significantly increased when the cells were treated with OxLDL, but not with native LDL or acetylated LDL. Production of PGE 2 by Ca9‐22 cells was enhanced by co‐incubation with OxLDL and interleukin‐1β (IL‐1β). Scavenger receptor inhibitors, fucoidan and dextran sulfate, inhibited the OxLDL‐induced IL‐8 and PGE 2 production in the presence of IL‐1β. The p 38 MAPK inhibitors SB203580 and SB202190 and the ERK inhibitor PD98059 inhibited the OxLDL‐induced IL‐8 production. Among oxidized lipids and chemically modified LDL, 7‐ketocholesterol enhanced IL‐8 production. Conclusion: This is the first report to show that OxLDL enhances IL‐8 production in epithelial cells.