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Hemostatic effects of fibrinogen γ‐chain dodecapeptide‐conjugated polymerized albumin particles in vitro and in vivo
Author(s) -
Okamura Yosuke,
Takeoka Shinji,
Teramura Yuji,
Maruyama Hitomi,
Tsuchida Eishun,
Handa Makoto,
Ikeda Yasuo
Publication year - 2005
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.2005.00173.x
Subject(s) - fibrinogen , conjugated system , in vivo , chemistry , platelet , in vitro , thrombus , albumin , polymerization , biochemistry , medicine , surgery , immunology , organic chemistry , polymer , biology , microbiology and biotechnology
BACKGROUND: Prototypes of platelet (PLT) substitutes have been studied and the focus was on a dodecapeptide, HHLGGAKQAGDV (H12), which is a fibrinogen γ‐chain carboxy‐terminal sequence (γ 400‐411) and exists only in the fibrinogen domain. STUDY DESIGN AND METHODS: H12 was conjugated to the surface of polymerized albumin particles (polyAlb) as biocompatible and biodegradable particles with a mean diameter of 260 ± 60 nm, and the hemostatic ability of H12‐conjugated polyAlb (H12‐polyAlb) under flow conditions and thrombocytopenic rats have been studied. RESULTS: H12‐polyAlb enhanced the in vitro thrombus formation of activated PLTs on a collagen‐immobilized plate when exposed to the flowing thrombocytopenic imitation blood. Furthermore, the analysis of the tail bleeding time of rats that were made thrombocytopenic by busulfan injection showed that H12‐polyAlb had a hemostatic effect. Based on the bleeding time and the amount injected, the hemostatic capacity of 20 H12‐polyAlb was estimated to correspond to that of one PLT. CONCLUSION: These results were important first steps toward the development of PLT substitutes and indicated that H12‐polyAlb may be a suitable candidate for an alternative to human PLT concentrates transfused into thrombocytopenic patients in the future.