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Pyruvate Improves Neutrophilic Nitric Oxide Generation in Peritoneal Dialysis Solutions
Author(s) -
Wu YiTai,
Wu ZhaoLong,
Jiang XiaoFeng,
Li Su,
Zhou FangQiang
Publication year - 2005
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/j.1525-1594.2005.00167.x
Subject(s) - peritoneal dialysis , nitric oxide , chemistry , salt (chemistry) , nitrite , biochemistry , nuclear chemistry , dialysis , inorganic chemistry , pharmacology , medicine , organic chemistry , nitrate
Abstract: To investigate the effects of pyruvate (Pyr)‐based peritoneal dialysis solutions (P‐PDS) on neutrophilic nitric oxide (NO) generation, we incubated human peripheral neutrophils in dL ‐lactate (Lac, 40 mM)‐based PDS and equimolar P‐PDS, and Hanks’ balanced salt solution at various pH and high glucose (HG) levels, respectively. The production of NO in various test solutions was determined based on the Griess reaction. Acidic pH, high Lac, and HG induced an acute and substantial inhibition of neutrophilic NO, whereas Pyr in PDS significantly improved the NO generation in both acidic pH and physiological pH, and also in HG conditions. The Pyr protection may be associated with the improvement of glucose metabolic pathways in addition to its alkalization.