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Carotenoids’ influence on radiotolerance of Pantoea agglomerans , a plant pathogen
Author(s) -
Dussault D.,
Caillet S.,
Le Tien C.,
Lacroix M.
Publication year - 2008
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2008.02410.x
Subject(s) - pantoea agglomerans , carotenoid , food science , antioxidant , microbiology and biotechnology , chemistry , strain (injury) , bacteria , biology , biochemistry , genetics , anatomy
Aim: The aim of this study was to evaluate the effect of γ radiation on the carotenoid content of two strains of the Enterobacteriaceae : Pantoea agglomerans . Methods and Results: Pantoea agglomerans strains ATCC 49174 and RL1 were used for this study. Successive radiation treatments were performed to study the radiotolerance. Total carotenoids were obtained by multiple extraction using chloroform/methanol (2 : 1), quantified by measuring the optical density at 453 nm and their antioxidant activity measured by a colorimetric method. The D 10 studies were conducted using a UC‐15A irradiator loaded with 60 Co. Bacterial counts from various dilutions were carried out after irradiation. Strain ATCC 49174 irradiated at 1 kGy produced 4·3 times more carotenoids than the control, whereas carotenoid synthesis increased by 2·9‐fold in the strain RL1. However, there was no significant difference in the D 10 values. Conclusion: Carotenoid increased production is influenced by γ radiation but does not modify the tolerance to radiations. Significance and Impact of the Study: To our knowledge, this is the first study to demonstrate the effects of γ radiation on carotenoid production levels.