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A set of primers conserved in genus Parnassius (Lepidoptera, Papilionidae) for amplification and sequencing of 1016 bp fragment of cytochrome oxidase subunit I from museum specimens
Author(s) -
KONOPINSKI MACIEJ K.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.02045.x
Subject(s) - biology , lepidoptera genitalia , mitochondrial dna , cytochrome c oxidase subunit i , intraspecific competition , amplicon , zoology , cytochrome b , genus , genetics , microbiology and biotechnology , botany , polymerase chain reaction , gene
Four short, overlapping amplicons covering a 1016 bp fragment of mitochondrial cytochrome oxidase subunit I were developed. All four fragments were successfully amplified and sequenced in eight species of butterflies belonging to the genus Parnassius including over 100‐year‐old DNA from pinned museum specimens. The fragment contains sufficient variation for both inter‐ and intraspecific analyses. A total of 105 sites were polymorphic within 52 haplotypes found in 186 samples from Parnassius mnemosyne .