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Development of 16 microsatellite markers for the European cockchafer, Melolontha melolontha
Author(s) -
ENKERLI JÜRG,
GISLER ALEXANDRA,
KÖLLIKER ROLAND,
WIDMER FRANCO
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01909.x
Subject(s) - biology , microsatellite , allele , population , genetics , pest analysis , zoology , evolutionary biology , botany , gene , demography , sociology
Sixteen polymorphic microsatellite markers were isolated from the European cockchafer, Melolontha melolontha L., based on enriched genomic libraries. Allelic variability was assessed in a collection of 30 individuals obtained from a field in eastern Switzerland. The number of detected alleles ranged from three to 13 with a mean of 6.69 and average observed and expected heterozygosities were 0.52 and 0.66, respectively. The 16 highly polymorphic microsatellite markers represent a powerful molecular tool, which will allow for detailed population genetic analyses on this important pest insect.