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Isolation and characterization of 39 microsatellite loci in the endangered Japanese loach Leptobotia curta
Author(s) -
WATANABE KATSUTOSHI,
TAKESHIMA HIROHIKO,
IWATA AKIHISA,
ABE TSUKASA,
UEHARA KAZUHIKO,
KAKIOKA RYO,
KIHIRA DAIJIRO,
NISHIDA MUTSUMI
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01902.x
Subject(s) - biology , microsatellite , locus (genetics) , loss of heterozygosity , endangered species , genetic diversity , zoology , population , genetics , allele , evolutionary biology , ecology , gene , demography , sociology , habitat
A microsatellite‐enriched genomic library was obtained for the endangered Japanese loach Leptobotia curta , and 39 dinucleotide markers were successfully isolated and characterized. These markers had between one and nine alleles, with expected heterozygosity ranging from 0 to 0.839, in a population from the Lake Biwa–Yodo River system of Japan. Linkage equilibrium was observed in most loci, and only one locus showed significant deviation from Hardy–Weinberg equilibrium. These microsatellite markers will be useful for genetic diversity studies of wild and captive L. curta populations.