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[ 3 H] Diazepam Displacing Activity in Human Cerebrospinal Fluid
Author(s) -
Kuhn Wilfried,
Neuser Dieter,
Przuntek Horst
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb04494.x
Subject(s) - cerebrospinal fluid , diazepam , neuroscience , chemistry , psychology , medicine , pharmacology
Pooled human cerebrospinal fluid was separated by Sephadex G‐50 chromatography. The presence of three peaks, A, B and C, was demonstrated by monitoring absorbance at 254 and 280 nm. All peaks showed [ 3 H]diazepam displacing activity in the membrane receptor test. Peak B was further separated on Bio‐Gel P‐4. At least two major fractions free of salt and GABA in the molecular weight range of approximately 700‐3600 were shown to displace [ 3 H]diazepam in the receptor test. This activity was enhanced by a factor of 3 in the presence of 10 μ m ‐GABA.