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Differential Uptake of Horseradish Peroxidase Isoenzymes by Cultured Neuroblastoma Cells
Author(s) -
Chan Kwan Y.,
Haschke Richard H.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb02420.x
Subject(s) - horseradish peroxidase , isozyme , peroxidase , axoplasmic transport , intracellular , chemistry , selectivity , neuroblastoma , biochemistry , enzyme , microbiology and biotechnology , biophysics , biology , cell culture , catalysis , genetics
The endocytotic uptake and intracellular decay of horseradish peroxidase isoenzymes C and A by cultured mouse neuroblastoma cells were analyzed quantitatively by a direct spectrophotometric assay. At concentrations below 1 mg/ml, the rate of uptake of the isoenzyme C was more than three times as much as the isoenzyme A. This differential uptake suggests that previous claims of horseradish peroxidase being endocytosed only in the nonselective fluid phase are oversimplified. The implication of this selectivity in the biological significance of retrograde axonal transport of proteins by neuronal systems is discussed.