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Carbohydrate ingestion attenuates the increase in plasma interleukin‐6, but not skeletal muscle interleukin‐6 mRNA, during exercise in humans
Author(s) -
Starkie R. L.,
Arkinstall M. J.,
Koukoulas I.,
Hawley J. A.,
Febbraio M. A.
Publication year - 2001
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2001.0585a.x
Subject(s) - interleukin 1β , ingestion , skeletal muscle , endocrinology , carbohydrate , interleukin , medicine , messenger rna , interleukin 2 , interleukin 6 , chemistry , cytokine , biochemistry , gene
The present study was undertaken to examine the effects of exercise and carbohydrate (CHO) ingestion on interleukin‐6 (IL‐6) gene expression in skeletal muscle and plasma IL‐6 concentration. Seven moderately trained men completed 60 min of exercise at a workload corresponding to each individual's lactate threshold on four randomised occasions. Two trials were conducted on a bicycle ergometer (Cyc) and two on a running treadmill (Run) either with (CHO) or without (Con) the ingestion of a CHO beverage throughout the exercise. Muscle biopsies were obtained from the vastus lateralis before and immediately after exercise and IL‐6 gene expression in these samples was determined using real‐time PCR. In addition, venous blood samples were collected at rest, and after 30 min during and at the cessation of exercise. These samples were analysed for plasma IL‐6. Irrespective of exercise mode or CHO ingestion, exercise resulted in a 21 ± 4‐fold increase ( P < 0.01; main exercise effect) in IL‐6 mRNA expression. In contrast, while the mode of exercise did not affect the exercise‐induced increase in plasma IL‐6, CHO ingestion blunted ( P < 0.01) this response. These data demonstrate that CHO ingestion attenuates the plasma IL‐6 concentration during both cycling and running exercise. However, because IL‐6 mRNA expression was unaffected by CHO ingestion, it is likely that the ingestion of CHO during exercise attenuates IL‐6 production by tissues other than skeletal muscle.