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TNF‐α and IL‐10 gene polymorphisms show a weak association with pemphigus vulgaris in the Slovak population
Author(s) -
Javor J,
Chmurova N,
Parnicka Z,
Ferencik S,
GrosseWilde H,
Buc M,
Svecova D
Publication year - 2010
Publication title -
journal of the european academy of dermatology and venereology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.655
H-Index - 107
eISSN - 1468-3083
pISSN - 0926-9959
DOI - 10.1111/j.1468-3083.2009.03260.x
Subject(s) - medicine , pemphigus vulgaris , slovak , association (psychology) , gene , dermatology , immunology , genetics , biology , philosophy , linguistics , czech , epistemology
Abstract Backround Pemphigus vulgaris is a rare chronic autoimmune disease of skin and mucous membranes, with several cytokines participating in its development. The role of their gene polymorphisms in susceptibility to the disease is, however, not fully understood. Objective The aim of our case‐control study was to investigate whether some of 22 single nucleotide polymorphisms (SNPs) in 13 cytokine genes ( IL‐1α , IL‐1β , IL‐1RI , IL‐1Ra , IL‐4Rα , IL‐12 , IFN‐γ , TGF‐β1 , TNF‐α , IL‐2 , IL‐4 , IL‐6 and IL‐10 ) are associated with pemphigus vulgaris in the Slovak population. Methods DNA samples were obtained from 34 pemphigus vulgaris patients and 140 healthy controls of Slovak origin. Cytokine gene SNPs were determined using the polymerase chain reaction with sequence‐specific primers (PCR‐SSP) method. Results We found a weak association between pemphigus vulgaris and polymorphic variants in TNF‐α and IL‐10 genes only, with haplotypes TNF‐α–308G/–238G and IL‐10 –1082A/–819C/–592C being significantly overrepresented in pemphigus vulgaris patients (TNF‐α GG: 94.12% vs. 82.86%, P = 0.0216; IL‐10 ACC: 44.12% vs. 30.00%, P = 0.0309). Conclusions Our preliminary results suggest that certain TNF‐α and IL‐10 gene polymorphisms might contribute to genetic susceptibility to pemphigus vulgaris; however, their overall impact on disease development will be rather limited.