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Gene expression profiling in the human middle cerebral artery after cerebral ischemia
Author(s) -
Vikman P.,
Edvinsson L.
Publication year - 2006
Publication title -
european journal of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.881
H-Index - 124
eISSN - 1468-1331
pISSN - 1351-5101
DOI - 10.1111/j.1468-1331.2006.01496.x
Subject(s) - middle cerebral artery , downregulation and upregulation , ischemia , medicine , gene expression , immunohistochemistry , brain ischemia , gene expression profiling , real time polymerase chain reaction , pathology , microarray , rhoa , microarray analysis techniques , gene , biology , microbiology and biotechnology , signal transduction , genetics
We have investigated the gene expression in human middle cerebral artery (MCA) after ischemia. Ischemic stroke affects the perfusion in the affected area and experimental cerebral ischemia results in upregulation of vasopressor receptors in the MCA leading to the ischemic area. We obtained human MCA samples distributing to the ischemic area, 7–10 days post‐stroke. The gene expression was examined with real‐time polymerase chain reaction (PCR) and microarray, proteins were studied with immunohistochemistry.We investigated genes previously shown to be upregulated in animal models of cerebral ischemia (e.g. ET A , ET B , AT 1 , AT 2 , and 5‐HT 2A/1B/1D ). Their mRNA expression was increased compared with controls, consistent with findings in experimental stroke. Immunohistochemistry showed upregulation of the receptors localized on the smooth muscle cells. The gene expression was profiled with microarray and seven genes chosen for further investigation with real‐time PCR; ELK3 , LY64 , Metallothionin IG , POU3F4 , Actin α2 , RhoA and smoothelin . Six of these were regulated the same way when confirming array expression with real‐time PCR. Gene expression studies in the human MCA leading to the ischemic region is similar to that seen after MCA occlusion in rats. We found new genes that support the dynamic changes that occur in the MCA distributing to the ischemic region.