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X tr, a plural tudor domain‐containing protein, is involved in the translational regulation of maternal m RNA during oocyte maturation in X enopus laevis
Author(s) -
Ohgami Hiroki,
Hiyoshi Masateru,
Mostafa Md. Golam,
Kubo Hideo,
Abe ShinIchi,
Takamune Kazufumi
Publication year - 2012
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2012.01367.x
Subject(s) - oocyte , germinal vesicle , microbiology and biotechnology , biology , xenopus , immunoprecipitation , messenger rna , metaphase , chemistry , genetics , gene , embryo , chromosome
Xtr in the fertilized eggs of X enopus has been demonstrated to be a member of a messenger ribonucleoprotein (m RNP ) complex that plays a crucial role in karyokinesis during cleavage. Since the X tr is also present both in oocytes and spermatocytes and its amount increases immediately after spematogenic cells enter into the meiotic phase, this protein was also predicted to act during meiotic progression. Taking advantage of X enopus oocytes’ large size to microinject anti‐ X tr antibody into them for inhibition of X tr function, we examined the role of X tr in meiotic progression of oocytes. Microinjection of anti‐ X tr antibody into immature oocytes followed by reinitiation of oocyte maturation did not affect germinal vesicle break down and the oscillation of Cdc2/cyclin B activity during meiotic progression but caused abnormal spindle formation and chromosomal alignment at meiotic metaphase I and II. Immunoprecipitation of X tr showed the association of X tr with FRGY 2 and m RNA s such as RCC 1 and XL ‐ INCENP m RNA s, which are involved in the progression of karyokinesis. When anti‐Xtr antibody was injected into oocytes, translation of XL ‐ INCENP m RNA , which is known to be repressed in immature oocytes and induced after reinitiation of oocyte maturation, was inhibited even if the oocytes were treated with progesterone. A similar translational regulation was observed in oocytes injected with a reporter m RNA , which was composed of an enhanced green fluorescent protein open reading frame followed by the 3′ untranslational region (3′ UTR ) of XL ‐ INCENP m RNA . These results indicate that X tr regulates the translation of XL ‐ INCENP m RNA through its 3′ UTR during meiotic progression of oocyte.