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Mitochondrial DNA Polymorphism and Determination of Effects on Reproductive Trait in Pigs
Author(s) -
Yen NT,
Lin CS,
Ju CC,
Wang SC,
Huang MC
Publication year - 2007
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00797.x
Subject(s) - single strand conformation polymorphism , mitochondrial dna , biology , offspring , polymorphism (computer science) , genetics , trait , zoology , gene , polymerase chain reaction , genotype , pregnancy , programming language , computer science
Contents The purposes of this study were to investigate single strand conformation polymorphisms (SSCP) in the D‐loop region of pig mitochondrial DNA (mtDNA) and to determinate their association with the reproductive traits of meishan pigs. A total of four types of band patterns, designed SSCP band pattern A, B, C and D, were identified. A type of SSCP band pattern was present in all European–American breeds, but not in East Asian breeds. This result showed the diversified sequence in the D‐loop region between European–American and East Asian populations. Two types of band patterns, B and C, were found in Meishan pigs. The average body weight at day 21 of piglets from B type dams was significantly heavier than the body weight of C type (p < 0.05). We also tested whether the SSCP patterns would be suitable for paternity testing in a family group and found that bands of all the offspring were derived from their maternal parent. Therefore, we conclude that SSCP may be a marker for identification of maternal ancestors.