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Identifizierung von Sporothrix schenckii mittels Sequenzierung des Chitinsynthase‐1‐Gens
Author(s) -
Kano R.,
Nakamura Y.,
Watanabe S.,
Tsujimoto H.,
Hasegawa A.
Publication year - 2001
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2001.00655.x
Subject(s) - sporothrix schenckii , sporotrichosis , chitin synthase , ethidium bromide , biology , genomic dna , polymerase chain reaction , primer (cosmetics) , sporothrix , gene , microbiology and biotechnology , dna , chitin , genetics , chemistry , biochemistry , immunology , organic chemistry , chitosan
Summary. Sporothrix schenckii is pathogenic to human and animals. To detect S. schenckii in the tissue, we designed specific oligonucleotide primers based on the chitin synthase 1 gene. Amplification products were selectively obtained from S. schenckii DNA. Polymerase chain reaction analysis with the primer pair S2–R2 was able to detect 10 pg genomic DNA of S. schenckii with ethidium bromide staining. This detection system will be useful as a microbiological tool for the diagnosis of human and animal sporotrichosis.