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Induced ovulation in cultured tawny puffer Takifugu flavidus by injections of LHRH‐a
Author(s) -
Shi Y.,
Zhang G.,
Liu J.,
Zhu Y.,
Zhang H.
Publication year - 2012
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.2011.01780.x
Subject(s) - ovulation , biology , human fertilization , zoology , saline , body weight , fish <actinopterygii> , andrology , significant difference , endocrinology , medicine , hormone , fishery , anatomy
Summary To determine the effective LHRH‐a dosage for cultured tawny puffer ovulation, the effects of 10, 30, 50, and 70 μg kg −1 body weight (BW) LHRH‐a were tested with two injections in 2009, and by a single injection (5, 10, 30, 50, and 70 μg kg −1 BW) in 2010. Ovulation success and egg quality were assessed. All females spawned after being treated with 10 to 70 μg kg −1 BW LHRH‐a in 2009 and 2010. Ovulation success was only 20.0% with the 5 μg kg −1 BW treatment, significantly lower (P < 0.05) than in the other treatment groups. No females injected with physiologic saline spawned in the control groups in 2009 and 2010. There was no significant difference (P > 0.05) among the 10, 30, 50, and 70 μg kg −1 BW treatment groups in terms of ovulation success, ovulation time, egg production, fertilization rate, hatch rate, and viability of 24 h post‐hatch larvae in 2009 and 2010. None of the treatments caused any mortality among the females during the experimental periods. Results suggest that the treatment using only LHRH‐a effectively and reliably induces ovulation in cultured tawny puffer broodstocks, whether through a single injection or through two injections. Effective and most economical dosage of LHRH‐a is 10 μg kg −1 BW. Determination of the effective LHRH‐a dosage is an important step in the development of tawny puffer fish culture.