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The coding sequence of the ASIP gene is identical in nine wild‐type coloured cattle breeds
Author(s) -
Royo L.J.,
Álvarez I.,
Fernández I.,
Arranz J.J.,
Gómez E.,
Goyache F.
Publication year - 2005
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.2005.00541.x
Subject(s) - coding region , exon , biology , genetics , gene , genotype , single strand conformation polymorphism , locus (genetics) , coat , breed , dna sequencing , polymerase chain reaction , paleontology
Summary The aim of this study was to ascertain the role of the Agouti signaling peptide (ASIP) gene coding region in the Agouti locus variation within wild‐type coat colour in cattle. We determined the Extension genotype in 241 individuals from six Spanish and three French brown cattle breeds representative of wild‐type coat variation. Polymerase chain reaction–single‐strand conformation polymorphism (PCR‐SSCP) analysis was carried out in individuals of each Extension genotypes within the same breed in an attempt to identify variants in the three coding exons of the ASIP gene. No SSCP variants were found. Results were confirmed by sequencing the coding exons of the ASIP gene in 20 individuals. Our results suggest that the ASIP coding region does not play a central role in coat colour variation in cattle.