Open AccessStructural Studies of the O‐Specific Polysaccharide of Hafnia alvei Strain PCM 1206 Lipopolysaccharide Containing D‐Allothreonine
Author(s) -
Petersson Carl,
Niedziela Tomasz,
Jachymek Wojciech,
Kenne Lennart,
Zarzecki Przemyslaw,
Lugowski Czeslaw
Publication year - 1997
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1997.00580.x
Subject(s) - polysaccharide , amide , hafnia , strain (injury) , chemistry , hydrolysis , lipopolysaccharide , nuclear magnetic resonance spectroscopy , stereochemistry , biochemistry , organic chemistry , biology , ceramic , cubic zirconia , anatomy , endocrinology
The structure of the O‐specific side‐chain of the Hafnia alvei strain PCM 1206 lipopolysaccharide has been investigated. Methylation analysis, partial acid hydrolysis, FAB‐MS/MS and 1 H‐NMR and 13 C‐NMR spectroscopy were the principal methods used. d ‐Allothreonine ( d ‐aThr), amide‐linked to the d ‐galacturonic acid, was identified as a constituent in the polysaccharide and the following structure of a pentasaccharide repeating unit was established:
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