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A novel device detects a rapid ozone‐induced transient stomatal closure in intact Arabidopsis and its absence in abi2 mutant
Author(s) -
Kollist Triin,
Moldau Heino,
Rasulov Bahtijor,
Oja Vello,
Rämma Heikko,
Hüve Katja,
Jaspers Pinja,
Kangasjärvi Jaakko,
Kollist Hannes
Publication year - 2007
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2006.00851.x
Subject(s) - guard cell , cuvette , abscisic acid , biophysics , arabidopsis , stomatal conductance , mutant , chemistry , plant cell , conductance , botany , microbiology and biotechnology , biochemistry , biology , photosynthesis , gene , physics , mathematics , quantum mechanics , combinatorics
To follow stomatal responses to ozone (O 3 ) in different Arabidopsis lines, we constructed a rapid‐response O 3 exposure/gas‐exchange measurement device consisting of eight through‐flow whole‐rosette cuvettes. To separate rosette from roots and growth substrate, plant is grown through an agar‐filled hole in a polished glass plate fixed on the pot. Following insertion of the plant, the plate forms air‐tight bottom surface of the cuvette; thus the rosette is enclosed without touching it during any phase of the insertion of the plant to the cuvette. The device allows monitoring rapid responses in the stomatal function. For example, an acute exposure to 150 ppb O 3 decreased stomatal conductance to 60–70% of its initial value within 9–12 min. Thereafter, the conductance regained its preexposure value within further 30–40 min in spite of the continuing O 3 exposure. The transient decrease was absent in the abscisic acid‐insensitive mutant abi2 defective in a class 2C protein phosphatase. This provides an in vivo confirmation that the early transient decrease in stomatal conductance is not a result of physical damage by the reactive oxygen species (ROS) formed from O 3 breakdown but reflects the biological action of ROS, transduced through a signalling cascade. Thus, the apparatus will be helpful in specifying complex molecular and genetic interactions in rapid responses in guard cells in vivo.