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The identification of Y chromosome translocations following Distamycin A treatment
Author(s) -
Cohen Maimon M.,
Frederick Robert W.,
Balkin Nancy E.,
Simpson Stacy J.
Publication year - 1981
Publication title -
clinical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 102
eISSN - 1399-0004
pISSN - 0009-9163
DOI - 10.1111/j.1399-0004.1981.tb00720.x
Subject(s) - chromosomal translocation , biology , chromosome , genetics , karyotype , heterochromatin , phenotype , microbiology and biotechnology , lymphocyte , gene
Pretreatment of PHA‐stimulated lymphocyte cultures from normal males with 100 (μg/ml Distamycin A for the final 24 h of incubation led to a striking decondensation of the heterochromatic portion of the long arm of the Y chromosome. The despiralized region of Yq maintained its fluorescent properties in this altered morphologic state. Two cases of Y chromosome translocation (Y/22 and Y/X) were identified with this technique. A review of the literature revealed 62 similar cases in which the phenotypic‐karyotypic correlations were instrumental in the prenatal counselling of the family with the Y/22 translocation.
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