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Metronidazole: a method for its determination in biological fluids and its disposition kinetics in the dog
Author(s) -
NEFFDAVIS CAROL A.,
DAVIS LLOYD E.,
GILLETTE EDWARD L.
Publication year - 1981
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1981.tb00720.x
Subject(s) - metronidazole , bioavailability , pharmacokinetics , pharmacology , chemistry , volume of distribution , chromatography , absorption (acoustics) , elimination rate constant , oral administration , distribution volume , antibiotics , medicine , biochemistry , physics , acoustics
A method for the analytical determination of metronidazole concentrations in biological tissues was developed using high performance liquid chromatography. The procedure was employed to investigate the pharmacokinetics of metronidazole in dogs following intravenous and oral administration (44 mg/kg). The overall elimination rate constant β was 0.0027 ± 0.0005 min ‐1 , the apparent specific volume of distribution (V' d ) was 0.948 ± 0.096 L/kg overall clearance (Cl B ) was 2.49 ± 0.54 ml/kg/min and the rate constant for absorption K ab was 0.0456 ± 0.0353 min ‐1 . Oral bioavailability was high but variable (59%–100%). Implications of these data for chemotherapy of infections caused by anaerobic bacteria, trichomonads, and Giardia and for the sensitization of hypoxic neoplastic cells to radiotherapy are discussed.