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Establishment of a cell line of human skin squamous cell carcinoma in vitro
Author(s) -
KONDO S.,
ASO K.
Publication year - 1981
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1981.tb01196.x
Subject(s) - basal cell , in vitro , cell culture , medicine , oncology , human skin , cancer research , dermatology , pathology , biology , genetics
SUMMARY A cell line of a squamous cell carcinoma of human skin (HSC‐1) has been established in vitro and successfully maintained prolifcrativc in continuous tissue culture for ahout 2 1/2 years since March 1978. Another cell line (HSC‐1b) has been established in vitro from the recurrence of the same cancer. It has been maintained for about 8 months since December, 1979. 3T3 feeders were used in the initial cultures in both cases. The cells grow in a monolayer in vitro and are epithelioid with anaplastic features. Chromosome analysis of HSC‐i, frozen and thawed HSC‐1, the transplanted tumour in a nude mouse and HSC‐1b all showed hypotetraploidy with the modal number 80, which reconfirms that HSC‐1 derives from the original skin tumour. The cell line is available to other investigators.