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Towards standardization of canine STRs: a proposed nomenclature for six markers from the ISAG comparison‐test panel
Author(s) -
Ciampolini R.,
Cecchi F.,
Bramante A.,
Tancredi M.,
Presciuttini S.
Publication year - 2012
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2011.02267.x
Subject(s) - biology , microsatellite , genetics , allele , nomenclature , allele frequency , population , breed , computational biology , evolutionary biology , zoology , gene , medicine , taxonomy (biology) , environmental health
Summary Eight short tandem repeat markers included in the International Society for Animal Genetics panel of 24 loci investigated in canine comparison tests were analysed in a sample of pure‐breed dogs, with the purpose of defining an allele nomenclature based on the number of repeats. A regression analysis of the raw data produced by the sequencer, coupled with the direct sequencing of selected alleles, allowed us to propose a system of nomenclature for six of the eight loci (four di‐nucleotidic: AHT121 , AHTh137 , REN169018 and REN64E19 , and two tetra‐nucleotidic: FH2001 and FH2328 ). The remaining two loci ( INU055 and FH2848 ) showed a pattern of fragments that did not resolve in a simple allele series. This work may be useful to establish a basis for comparing data across different laboratories for a set of validated canine markers, which can be used in population genetics, forensics and other analyses.